APA
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Zheng, S., Chiang, Y.-H., Aldarondo, D., Fox, Z. R., Akpa, B. S., & Wayne, E. (2026). Capturing the dynamics of STAT6 macrophage polarization using bioluminescence temporal spectrometry. Cell Systems, Accepted(https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4682259).
Chicago/Turabian
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Zheng, Shiyuan, Yi-Hsuan Chiang, Dasia Aldarondo, Zachary R Fox, Belinda S Akpa, and Elizabeth Wayne. “ Capturing the Dynamics of STAT6 Macrophage Polarization Using Bioluminescence Temporal Spectrometry.” Cell Systems Accepted, no. https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4682259 (2026).
MLA
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Zheng, Shiyuan, et al. “ Capturing the Dynamics of STAT6 Macrophage Polarization Using Bioluminescence Temporal Spectrometry.” Cell Systems, vol. Accepted, no. https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4682259, 2026.
BibTeX Click to copy
@article{shiyuan2026a,
title = { Capturing the dynamics of STAT6 macrophage polarization using bioluminescence temporal spectrometry},
year = {2026},
issue = {https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4682259},
journal = {Cell Systems},
volume = {Accepted},
author = {Zheng, Shiyuan and Chiang, Yi-Hsuan and Aldarondo, Dasia and Fox, Zachary R and Akpa, Belinda S and Wayne, Elizabeth}
}
Alternatively activated macrophage or M2 macrophage, in response to inflammatory stimuli, plays a pivotal role in wound healing, immunosuppression, and tumor progression. STAT6-mediated signaling pathway, activated by interleukin 4(IL-4)/IL-13, drives the alternative macrophage polarization. STAT6 functions by forming a phosphorylated homodimer and binding onto STAT6-responsive elements within promoters to regulate anti-inflammatory associated genes. Measuring STAT6 activity can be a proxy for measuring macrophage polarization. However, cellular probes that enable real-time measurement of macrophage polarization are needed. Specifically, we developed a STAT6-RE THP-1 reporter to access STAT6 activation in response to inflammatory stimuli. We can quantitatively measure macrophage polarization with bioluminescence temporal spectrometry (BTS). In this study, wildtype THP-1 monocytes were transduced with lentivirus to express the STAT6-RE-FLuc-GFP. We demonstrate that the reporter is activated only by phosphorylated STAT6 via immunohistochemistry and ELISA. Notably, the STAT6-RE can accurately report the dynamics of endogenous STAT6 activity. The results indicated a novel probe that can assess macrophage alternative polarization. These experiments allow for a better understanding of the association between molecular mechanisms and macrophage polarization, facilitating the development of therapeutic strategies for manipulating macrophages to attenuate inflammation and tumor progression and promote wound healing.